Anticancer Efficacy of Chitosan Nanoparticles from Freshwater Crab Sartoriana spinigera: Insights into Apoptotic and Necrotic Pathways in MCF-7 Cells | Chapter 2 | Research Perspective on Biological Science Vol. 8

 

Background: Chitosan is an abundant polymer found in the exoskeleton of crustaceans, insects, molluscs and the cell wall of fungi. It is well known for its wound healing capacity, anti-oxidative efficacy, antimicrobial activity and more. Most chitosan extraction processes have focused on exoskeletons of marine organisms, while studies using freshwater sources remain limited.

 

Aims: The aim of the present study was to evaluate the in vitro anticancer efficacy of chitosan nanoparticles extracted from the carapace of freshwater crab Sartoriana spinigera by studying Caspase 3/7 and Annexin V concentration through apoptosis and necrosis percentage of MCF-7 Breast cancer cells.

 

Methodology: The study samples were prepared in the Department of Zoology, Marwari College, Ranchi University, and an in vitro anticancer study was done in Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India. Activity of caspase 3/7 indicator was studied using Caspase 3/7 green detection reagent after treatment with Chitosan Nanoparticles for 24 hours, and analysing the reading by flow cytometry. Annexin V was estimated by studying apoptosis and necrosis using Annexin V- FITC and Propidium iodide, and after treatment with Chitosan nanoparticles, analysis was done by flow cytometry. Statistical analysis was done by Student’s t-test.

 

Results: A Study on enhancing Caspase 3/7 activity of MCF-7 cells has been done by treatment with chitosan nanoparticles. At 262 µg/mL, early apoptosis of 7.78% and late apoptosis of 4.02% in MCF-7 cells took place. ChNP was able to show increased necrosis of MCF-7 cells, which was significantly higher than that of the control group at 5 % significance level. At 131 µg/mL, ChNP showed cell necrosis of 24.5% and at 262 µg/mL, cell necrosis of 27.3% was noted. Analysis of Annexin V revealed that chitosan nanoparticles at a concentration of 131 µg/mL increased the phenomenon of early apoptosis of 0.35% of MCF-7 cells and necrosis of 15.59%.

 

Conclusion: The present study confirms that chitosan nanoparticles extracted from freshwater crab Sartoriana spinigera is a potent anticancer agent against the MCF-7 cell line. This cost-effective treatment with minimal side effects can be considered as an anticancer drug. However, in vivo study and clinical trial need to be done to validate this reliability.

 

 

Author(s) Details

Shiny E.C. Kachhap
Department of Zoology, Marwari College, Ranchi University, Ranchi, India.

 

Nayni Saxena
University Department of Zoology, Ranchi University, Ranchi, India.

 

Please see the book here :- https://doi.org/10.9734/bpi/rpbs/v8/6318

 

Anticancer Activity of Adathoda vasica in Hela, HepG2, MCF-7, MDAMB-231 Cell Lines- In vitro| Chapter 7 | Current Overview on Pharmaceutical Science Vol. 2

 This study was begun to evaluate the effect o methanolic extract of the leaf of Adhatoda vasaka on various cancer container lines like MCF-7, MDA- MB-231, Hela, HepG2 cell lines. The ethanolic extract of Adhatoda vasica was proven for allure anticancer cytotoxic properties artificial on a variety of tumor cell lines, containing MCF-7, The active elements carbohydrates, steroids, Alkaloids, Tannins, Flavanoids, Saponins, gums and mucilage. The ethanolic extract of Adhatoda vasica demonstrated a good cytotoxic activity (60%, 60%, 85% and 65%) in Hela, HepG2, MCF-7, MDAMB-231 containers at 24 employment recruiting and management respectively. In this In-vitro studies the common cells are comprise a positive control. The ethanolic extract of Adhatoda vasica shows maximum allotment of 85% after 24 employment recruiting and management. From this study, it can be decided that the phytoconstituents of Adhatoda vasica have significant anticancer-cytotoxic endeavor on Hela, HepG2, MCF-7, MDAMB-231 containers. According to the findings of the current hearing, the Adhatoda vasica Methanolic extract showed antagonistic-cancer action only in the plant's leaf extract, which was favorable. At doses of 200 mg per kilogramme and 400 mg per kilogramme of body burden, Linn., leaf significantly prevents the growth of MCF-7, MDA MB-231, HeLa, and HepG2 containers.

Author(s) Details:

Sankaradoss Nirmala,
Department of Pharmacognosy, Faculty of Pharmacy, Sree Balaji Medical College and Hospital Campus, Bharath Institute of Higher Education and Research, Chromepet, Chennai-44, India.

P. Nadana Sabapathi,
Formulation R & D, Sunglow Lifesciences PVT Ltd, Chengalpet, India.

Y. Sravanthi,
Malla Reddy College of Pharmacy (Affiliated OU), Hyderabad-500014, Telangana, India.

Please see the link here: https://stm.bookpi.org/COPS-V2/article/view/9169

Interactions of Downregulated Proteins in MCF-7 Cells, Treated with Resveratrol | Chapter 1 | Current Practice in Medical Science Vol. 6

Breast cancer, the age-old disease, is still an unmet need for many people around the world with over 600,000 fatalities every year. The usage of natural substances, such as resveratrol, which is present in grapes and many other plants, is a feasible alternative that is easy on the body and the wallet when compared to the price and severe side effects of the regularly used chemotherapy medications. In order to do this, the goal of this study is to examine how the Using string interaction diagrams and the corresponding gene ontology functional enrichments and pathways, it was possible to identify proteins that were downregulated in MCF-7, the human oestrogen receptor positive cell line, after resveratrol treatment. The findings suggest that Resveratrol, with its many anticancer properties, could be a promising anticancer drug, and that more research is required in that direction.

 

Author (s) Details

Raji Sundararajan

Purdue University, West Lafayette, IN 47907, USA.

Ignacio G. Camarillo

Purdue University, West Lafayette, IN 47907, USA

 

View Book :-  https://stm.bookpi.org/CPMS-V6/article/view/7674

 

Synthesis and Antitumor Evaluation of Some New Derivatives and Fused Heterocyclic Compounds Derived from Thieno[2,3‐ b]pyridine: Recent Perspective | Chapter 1 | Current Research and Development in Chemistry Vol. 3

As a continuation of our research on developing anticancer agents and based on the proven
proprieties of thieno[2,3‐b]pyridines as anticancer, we have designed to synthesize novel thieno[2,3‐
b]pyridine derivatives that incorporate different biologically active heterocycles through various
chemical reactions. All of the newly obtained compounds, compared with the standard anticancer
drug (doxorubicin), were screened in vitro for their antitumor activity against hepatocellular carcinoma
(HepG‐2) and human breast cancer (MCF‐7) cell lines. The results revealed that compounds 3, 7, 12,
and 19 were found to be the most potent against both HepG‐2 and MCF‐7 cell lines exhibiting IC50
values ranging from 3.67 to 11.50 and 5.13 to 11.80 μg/mL, respectively, among which compound 7
has a more potent activity than the reference drug doxorubicin against HepG‐2 cell line, showing IC50
value of 3.67 μg/mL (doxorubicin 4.65 μg/mL).

Author(s) Details

Aisha Y. Hassan
Department of Chemistry, Faculty of Science (Girls), Al‐Azhar University, Cairo, Egyp

Samiha A. El‐Sebaey
Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy (Girls), Al‐Azhar University, Cairo, Egypt.

View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/217

Study on Antioxidant Activity and Anticarcinogenic Properties of “Rumput mutiara” {Hedyotis corymbosa (L.) Lam.} and “Pohpohan” {Pilea trinervia (Roxb.) Wight} | Chapter 9 | Current Research Trends in Biological Science Vol. 2

The research was conducted to determine the anticarcinogenic properties of “rumput mutiara” (Hedyotis corymbosa (L.) Lam) and “pohpohan” (Pilea trinervia (Roxb.) Wight), by the microculture tetrazolium salt (MTT) assay on the human breast carcinoma dependent-hormone (MCF-7) cell lines. The preliminary results showed that the “rumput mutiara” extract displayed the cytotoxic effects against MCF-7 with IC50-value of 22,67 µg/ml. However, the “pohpohan” extract did not show the IC50value against MCF-7 cell lines. The antioxidative activities of the extracts which could contribute to their cytotoxic properties were also studied. The “rumput mutiara” extract was found to have higher antioxidant activity compared with “pohpohan” extract. The strong cytotoxic properties of the “rumput mutiara” extract could be due to its high antioxidant activity. The combination effect of “rumput mutiara” and soursop were also studied.

Author(s) Details

Susi Endrini
Faculty of Medicine and Health Sciences, Universitas Abdurrab, Jl. Riau Ujung Pekanbaru Riau Indonesia.

Himmi Marsiati
Faculty of Medicine, Yarsi University, Cempaka Putih 10510, Jakarta, Indonesia.

View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/188