Reports of the Seroepidemiologic Survey for Human Sparganosis in Mto wa Mbu Division, Monduli District, Tanzania | Chapter 2 | Recent Advances in Science and Technology Research Vol. 6

 

Sparganum is a pseudophyllidean tapeworm of the genus Spirometra plerocercoid. Northern Tanzania has been confirmed to have human sparganosis. In order to detect anti-sparganum specific IgG antibodies in the serum of normal inhabitants of Mto wa Mbu, Monduli District, Tanzania, a seroepidemiologic survey was performed. Sera was screened for antisparganum antibodies using the Enzyme-Linked Immunosorbent Assay ( ELISA). The positive antisparganum antibody incidence was 116 (62.7 percent) in 185 subjects. Of these, 17 (9.2) were adult males, 80 (43.2%) were adult females, and 19 (10.3%) were children (< 18). Questionnaire data for all 116 positive ELISA residents showed that they had a history of consuming game meat and drinking water from running springs. The data showed that ELISA would be useful for the identification of infected cases among normal residents in endemic areas of sparganosis.

Author (s) Details

N. J. Kavana
Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O.Box 3019, Morogoro, Tanzania.

C. J. Kasanga
Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O.Box 3019, Morogoro, Tanzania.

A. A. Kassuku
Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O.Box 3019, Morogoro, Tanzania.

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Critical Study on Immunodetection of Porcine Red Blood Cell Containing Food Ingredients Using a Porcine-Hemoglobin-Specific Monoclonal Antibody |Chapter 11 | Current Research in Agricultural and Food Science Vol.1

Monoclonal antibody (mAb) 24C12 has been found to bind to a 12 kDa antigenic protein in the red
blood cell (RBC) of porcine blood. The purpose of this study was to determine the identity of this 12
kDa protein and consequently examine its potential as a marker for monitoring porcine RBCcontaining ingredients (PRBCIs) in foods. Proteomic techniques identified the 12 kDa antigenic
protein to be a monomer of the tetrameric hemoglobin molecule. Further heat-processing of spraydried PRBCIs diminishes its detectability. Whereas mAb 24C12-based indirect enzyme-linked
immunosorbent assay (iELISA) could detect 1% (v/v) or less of PRBCIs in raw and cooked ground
meats (beef, pork and chicken), the detection limits were 3 to 30 times higher for spiked cooked beef
and pork. The assay is effective for monitoring the presence of PRBCIs in foods to protect the billions
of people that avoid consuming blood. In situations where these PRBCIs are present as ingredients in
foods that have undergone further heat processing, the assay, however, may not be as sensitive
depending on the types of sample matrix, types of PRBCIs and the level of inclusion.

Author (s) Details

Jack A. Ofori
Department of Nutrition, Food and Exercise Sciences, 420 Sandels Building, Florida State University, Tallahassee, FL 32306-1493, USA.

Yun-Hwa P. Hsieh
Department of Nutrition, Food and Exercise Sciences, 420 Sandels Building, Florida State University, Tallahassee, FL 32306-1493, USA.

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