The goal of this
study is to see if Virgin Coconut Oil (VCO) can help rats recover from
atrazine-induced metabolic disruption. Adult male albino wistar rats weighing
180-200g were used in the study. They were randomly assigned to one of two
primary experimental groups (The test and recovery groups). In the test group,
35 rats were randomly divided into five sub-groups of seven rats each (n=7) and
treated as follows: Subgroup (SG) 1 was the normal control, receiving 10 ml/kg
of distilled water, SG 2 received 10 ml/kg of VCO, SG 3 received 123 mg/kg of
Atrazine (ATZ), SG 4 was the diabetes control that was left untreated, and SG 5
was the diabetic group that received 10 ml/kg of VCO. The animals in the test
group were given medication for two weeks before being slaughtered and blood
taken for examination. Thirty-five rats from the recovery group were divided
into five sub-groups of seven rats each (n=7) and treated as follows for these
two weeks: The control group received 10 ml/kg of distilled water, while SG 2
received 10 ml/kg of VCO and SG 3, 4, and 5 received 123 mg/kg of ATZ. The
animals were re-treated for recovery after 2 weeks as follows: SG 1 received
10ml/kg distilled water, SG 2 received 10ml/kg VCO, SG 3 received 123mg/kg ATZ,
SG 4 received 10ml/kg VCO, and SG 5 received 10ml/kg of distilled water. The
animals were slaughtered after two weeks, and their blood was collected for
study. Gluthatione (GSH), Superoxide Dismutase (SOD), and Catalase (CAT) levels
were considerably lower (p0.05) in the atrazine and diabetes groups as compared
to the normal control. GSH levels in the VCO recovery group were substantially
higher (p0.05) than in the ATZ group after recovery. Finally, ATZ poisoning
generated oxidative stress, but its withdrawal lowered stress considerably,
with a stronger impact after VCO delivery.
Author(S) Details
Olatunbosun Titilope
Helen
Department of
Physiology, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria.
Uka Emmanuel
Department of Biochemistry, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom
State, Nigeria.
Asuquo Etim Asuquo
Department of Physiology, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom
State, Nigeria.
Jessica David Utibe
Department of Physiology, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom
State, Nigeria.
Otanwa Oladunni Omolabake
Department of Biochemistry, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom
State, Nigeria.
Akwo-Nelson Violet
Department of Biochemistry, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom
State, Nigeria.
View Book:- https://stm.bookpi.org/CAPRD-V7/article/view/5417
