Comparison of Virulence Genes in Staphylococcus aureus and Moraxella catarrhalis Isolated from Respiratory Tract Illnesses at the Molecular Level | Chapter 6 | Research Advances in Microbiology and Biotechnology Vol. 5

 Patients accompanying pharyngeal infections the one sought situation at Babylon General Teaching Hospital, Al-Hilla Hospital, and the Private Clinic of the Supervisor provided a total of two hundred and four (204) samples for reasoning. Swabs of Moraxella catarrhalis were collected from community aged 5 to 80, accompanying one set secondhand for culture and the additional set secondhand for direct DNA extraction. Ninety-three allotment (199) of the samples grew microorganisms in culture, while just six allotment (14 samples) did not. It was driven that 116/190 (61.10%) of the total isolates are Gram-positive microorganisms and 74/190 (38.90%) are Gram-negative bacteria from the 190 (100%) samples. Laboratory disease by biochemical tests, the Vitek-2 system, and microscopic detection by particular primers found that Staphylococcus aureus formed 38 (20%) of the 116 (100%) Gram-positive microorganisms, while Moraxella catarrhalis was the most governing with a portion of 44 (23.2%) of the 74 (100%) Gram-negative bacteria. Molecular study of S. aureus told that 13 strains, or 34%, possess the fnbA pathogenicity deoxyribonucleic acid. Regarding the M. catarrhalis mcaP gene, 44 exhausted 44 (100%) were positive. The fnbA deoxyribonucleic acid of S. aureus and the mcaP gene of M. catarrhalis, two together of which presented variation, were sequenced by DNA and before recorded in NCBI-deoxyribonucleic acid sequencing as initially characterized in Iraq. The adhesion genes fnbA and mcaP of grandam-positive S. aureus and grandam-negative M. catarrhalis were sequenced and analyzed utilizing DNA sequencing technology. The results revealed that local S. aureus isolates (NO. 111 and NO. 181) were closely had connection with a general S. aureus isolation recorded by NCBI-BLAST (AM749012.1), while local isolates (NO. 37 and NO. 95) were closely related to S. aureus strains from other parts of the realm (LC073768.1 and LC073762.1).

Author(s) Details:

Ilham A. Bunyan,
Department of Microbiology, College of Medicine, University of Babylon, Iraq.

Safaa S. Naji,
Department of Sergury, ENT, College of Medicine, University of Babylon, Iraq.

Hiader H. Aljodoa,
Ministry of Health, Babel Health Directorate, Al-Hilla General Teaching Hospital Laboratory Division, Babylon, Iraq

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Study on the Diversity of Heterotrophic Nitrogen Removal Bacteria in Piggery Wastes in the Mekong Delta, Vietnam | Chapter 7 | Innovations in Microbiology and Biotechnology Vol. 2

 The next phase was heterotrophic nitrogen removal in Pangasius fish (TRA) ponds in the Mekong Delta, now that we had the beneficial bacteria for wastewater treatment. A total of 2318 heterotrophic nitrogen removal (HNR) bacteria were isolated from piggery wastes (after biogas container) and divided into four categories: heterotrophic ammonia-oxidizing bacteria (569 isolates), nitrite-oxidizing bacteria (580 isolates), nitrate-oxidizing bacteria (600 isolates), and heterotrophic nitrifying and denitrifying bacteria (600 isolates) (569 isolates). The 16S rRNA gene was PCR amplified and sequenced almost completely. The HNR bacterium sequences were found to have a high degree of similarity to the GenBank reference strains (between 97 percent and 99.8 percent ). The consistency of phylogenetic trees based on 16S rDNA sequences was high, with nodes supported by high bootstrap (500) values. These suspected HNR isolates were split into four groups: Arthrobacter, Corynebacterium, Rhodococcus (high G+C content gram-positive bacteria), Staphylococcus, Bacillus (low G+C content gram-positive bacteria), and Klebsiella (low G+C content gram-positive bacteria) (gram-negative bacteria). In comparison to the other two groups, heterotrophic ammonium-oxidizing bacteria had the highest Pi values and heterotrophic nitrifying-denitrifying bacteria had the lowest Pi values. Theta values (per sequence) from S of SNP for DNA polymorphism showed that heterotrophic nitrate-oxidizing bacteria had the highest theta values. Many isolates had nitrogen utilisation ability at high concentrations (800–1200 mM), and high G+C gram-positive bacteria strains occupied greater than low G+C gram-positive bacteria strains, according to the current investigation on HNR bacteria from piggery wastes.

Author(S) Details

Cao Ngoc Diep
Biotechnology R&D Institute, Can Tho University, Vietnam.

Pham Viet Cuong
Vietnam Academy Institute of Science and Technology, Ha Noi City, Vietnam.

Bui The Vinh
CanTho Dairy Factory, Vietnam.

View Book:- https://stm.bookpi.org/IMB-V2/article/view/4719