Microsymbionts Isolated from Pea Root Nodules – the Study of Population Diversity | Chapter 2 | Current Research Trends in Biological Science Vol. 2

Aims: The study of strains belonging to local rhizobial population, concerning their diversity in the genetic, metabolic and symbiotic properties, and their prevalence in the microsymbiont population. Methodology: 257 rhizobial isolates recovered from nodules of five pea (Pisum sativum cv. Ramrod) plants grown at one site were classified using PCR-RFLP analysis of 16-23S rRNA ITS. After that, for representative group of 55 strains, 16-23S rRNA ITS region was sequenced, nodA-F region was analyzed by PCR-RFLP and sequencing, metabolic capabilities were studied using Biolog`s and growth tests and symbiotic performance in plant tests were assayed. Results: Individual plants were infected by numerous and diverse strains, however, in the entire sampled population of microsymbionts, only three large clusters of strains (one similar to Rhizobium pisi and two similar to Rhizobium leguminosarum) could be distinguished on the basis of PCR-RFLP and sequence analyses of 16S-23S rRNA ITS region. Rhizobium strains belonging to different groups varied in plasmid number and the amount of plasmid DNA, utilization of carbon and energy sources, growth on soil extract-based media and the ability for symbiotic plant growth promotion. The most numerous group of the isolates was characterized by the high plasmid DNA content, low number of utilized sugar substrates, and comprised numerous strains with low symbiotic efficiency. Conclusion: Sampled population of pea microsymbionts had its own characteristic structure with clearly distinguishable sub-populations, composed of numerous strains - probably descendants of a few old lineages, which diversified in the lapse of time. These strains are still competing during root nodule colonization, resulting in the symbiosis of individual pea plants with broad spectrum of different Rhizobium strains. 

Author(s) Details

Jerzy Wielbo
Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Akademicka 19 Street, 20-033 Lublin, Poland.

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Thripidae (Thysanoptera) Species Collected from Common Plants and Crops in Peninsular Malaysia: New Perspectives | Chapter 12 | Current Research in Science and Technology Vol. 4

A list of 66 species of thrips recorded from crops or plants in Peninsular Malaysia is presented. Most of the specimens came from a substantial survey of cultivated plants and one-third were from the genus Thrips. The specimens were collected by beating vegetation (that is, either the flowers or leaves) over a plastic tray, subjected to a standard dehydration and clearing process before being slide mounted. Slide mounted specimens were then identified using taxonomic keys. This study also recorded another twenty-one species of thrips besides the two former species from Malaysia: Frankliniella schultzei, a polyphagous tospovirus vector and Stenchaetothrips biformis, the rice thrips. The study reveals 66 species recorded as associated with cultivated plants in Malaysia; of these, 43 were found on more than one species of plant. 

Author(s) Details

 A. A. Azidah
Institute of Biological Sciences, Faculty of Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.

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