Ferula asafoetida Increases Cell Division and Cell Death in Vicia faba with Suppressing the Mitotic Index | Chapter 12 | Advanced Concepts in Pharmaceutical Research Vol. 5

Background: Homoeopathy is a system of medicine in which the indigenous and crude plant/ animal products are used in medicine. Extract of Ferula asafoetida (Heeng) is commonly used as Homoeopathic drug to cure various stomach related problems. There has been significant controversy associated with the side effects of Homoeopathic drugs.

Methodology: The mother tincture of Ferula asafoetida has been collected from RSBP Bulandshahar and Lord Pharmacy New Delhi, India. The effect of heeng as a Homoeopathic drug on somatic cell division has been studied by the root tip method. The effects of different concentrations of heeng on the chromosomes of Vicia faba was investigated. 5%, 10%, 20% & 30% of Ferula asafoetida were used for 4hrs, 8hrs, 12hrs and 24 hours followed by acetocarmine squash procedure. All the concentrations showed mito-inhibitory effect in the root tips of Vicia faba and also observed so many chromosomal aberrations (clastogenic).

Results: The treated root tips showed various disturbances at the prophase, metaphase and anaphase stages of mitotic cell division. Due to the stickiness of chromosomes, the separation of chromosomes was affected by the formation of chromatin bridges. The most observed aberrations were sticky chromosomes, chromatin bridges, chromatin fragments and laggards at every stage of mitotic division.

Conclusion: The result of the present study clearly established the cytogenotoxic behaviour of heeng as a Homoeopathic drug. The dose and duration should be tested for its various mutagenic and genotoxic properties in different eukaryotic systems viz. animals and humans.

Author(s) Details:

Sangeeta Dayal,
Department of Bio-Technology and Botany, KVSCOS, Swami Vivekanand Subharti University, Meerut (U.P.), India.

Alka Noopur,
ABN Classes, Sec-10, Raj Nagar, Ghaziabad (U.P.), India.

Harshal Kumar,
National Bureau of Plant Genetic Resources, IARI PUSA, New Delhi, India.

Please see the link here: https://stm.bookpi.org/ACPR-V5/article/view/13448

Oncofertility in the 21st Century: The Impact of Mutagenic Chemotherapy Exposure on Gamete Preservation | Chapter 07 | Issues and Developments in Medicine and Medical Research Vol. 6

 The influence of used drugs on hormonal and gonadal function, and the resulting diminished gonadal reserve or premature gonadal failure, are heavily entwined with cancer treatment-related infertility problems. While germ cell preservation recommendations are primarily focused on preventing cause infertility, they do not take into account earlier chemotherapeutic exposures that could result in genetic alteration, potentially leading to congenital abnormalities. This exploratory analysis is being presented in order to improve recognition of agents and exposures that should be identified during oncofertility assessment in order to prevent gamete preservation. The results of an original study analysing the established pharmacokinetics and mutagenic toxicities of conventional chemotherapy were compared to the rates at which such medicines were administered during a 23-week period. A significant 54 percent of the investigated agents (and related metabolites) were found to be mutagenic; analysis of the agent distribution pattern revealed mutagenic exposures in more than one-third of the treated child-bearing population, accounting for 27 percent of the total study patient population. Because more than half of conventional chemotherapeutics have the ability to modify the DNA of oocytes and sperm, past exposures to mutagenic chemotherapeutics should be investigated. If exposure is proven, gamete preservation may be prohibited due to an increased risk of genetic mutation, which could result in both physical and mental birth abnormalities. The necessity to examine newly emerging oncologic drugs for mutagenic/clastogenic potential, as well as the identification of such exposures in reproductive-age cancer patients, profoundly modifies the present approach to fertility preservation.

Author(S) Details

Nicole L. Longo, DO, FACOI
Board-certified in Internal Medicine, USA.

View Book:- https://stm.bookpi.org/IDMMR-V6/article/view/5599