Liver Transplantation Studies Using a Canine Model: A Zoom on the Intermediate Filaments of the Cytoskeleton of the Hepatocytes | Chapter 3 | Highlights on Medicine and Medical Research Vol. 9

 Liver transplantation can be used to treat both acute and chronic liver insufficiency. Graft malfunction persists in the twenty-first century. It affects up to one-third of those who receive it, despite reports of good to exceptional long-term outcomes. The etiologic examination of organ dysfunction revealed a complex basis at the time of authoring this chapter. Ischemia and reperfusion (I/R) injury is arguably the most major contributing factor for organ dysfunction, despite the numerous etiology. Cholestasis, or the cessation or reduction of bile flow with or without bile, is a condition in which the flow of bile is stopped or reduced. I/R damage is frequently accompanied with duct loss or ductopenia, as well as biliary ductular proliferations. They can, however, be deceiving, as they can also be present in other types of graft malfunction. Biliary marker levels often rise within 5 days of donation. The intermediate filaments of the cytoskeleton were studied in detail in this chapter using a liver transplantation model as an I/R damage model. The phenotypic flip of hepatocytes occurs before frank cholestasis, according to our findings. We believe that focusing on this phenotypic transformation in liver cells could aid in the savage or improved fate of the liver transplantation.

Author (s) Details

Consolato M. Sergi
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada and Stollery Children’s Hospital, University Alberta Hospital, Edmonton, AB, Canada.

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Allotransplantation Animal Study with Canine Model | Chapter 17 | Research Trends and Challenges in Medical Science Vol. 3

As reconstructive transplantation is gaining popularity as a viable alternative for upper limb amputees, it is becoming increasingly important for plastic surgeons to renew surgical skills and knowledge of this area. Forelimb allotransplantation research has been performed previously in rodent and swine models. However, preclinical canine forelimb allotransplantation studies are lacking in the literature. The purpose of this paper is to provide an overview of the surgical skills necessary to successfully perform forelimb transplantation in canines as ameans to prepare for clinical application. A total of 18 transplantation operations on canines were performed. The recipient limb was shortened at the onethird proximal forearm level. The operation was performed in the following order: bones (two reconstructive plates), muscles and tendons (separately sutured), nerves (median, ulnar, and radial nerve), arteries (two), and veins (two). The total mean time of transplantation was 5 hours ± 30 minutes. All of the animals that received transplantation were treated with FK-506 (tacrolimus, 2 mg/kg) for 7 days after surgery. Most allografts survived with perfect viability without vascular problems during the early postoperative period. The canine forelimb allotransplantation model is well qualified to be a suitable training model for standard transplantation and future research work.

Author(s) Details

Seokchan Eun
Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seoul 463-706, Republic of Korea.

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