Showing posts with label Real time PCR. Show all posts
Showing posts with label Real time PCR. Show all posts

Friday, 21 July 2023

Real Time PCR Based Diagnosis of Multiple Tick Transmitted Diseases in a Captive Asian Elephant-Elephas Maximus-A Case Report | Chapter 6 | Cutting Edge Research in Biology Vol. 9

 Real Time PCR located diagnosis of diversified tick transmitted pathogens in a captive Asian elephant (old 37 years) possessed by a temple in Thrissur District, Kerala State is documented. According to reports from the mosque authorities’ mammoth was not taking bread and water for past few days, had partial paralysis of container and progressive gauntness. Treatment was initiated but animal didn’t show some response to medicines secondhand and died next era. Peripheral blood in EDTA and peripheral ancestry smear was treated for confirmatory disease. Peripheral blood smear subsequently Giemsa staining revealed dot and ring like additions in the periphery and centre of RBC’s doubtful for inclusions of Anaplasma or Babesia species and morulae inside neutrophils signifying of Ehrlichia species or Anaplasma phagocytophilum. Real Time PCR established Taqman chemistry was done to validate multiple tick communicated pathogens. To authors knowledge this is the first report of RT PCR located diagnosis of multiple tick communicated pathogens in a captive elephant in united states of america of Kerala which endure be a major concern and necessitates predominance studies for the same with domesticated captive elephant public and treatment regimes has to be changed according to the findings and dossier generated.

Author(s) Details:

Sunitha Karunakaran,
Department of Animal Husbandry-Kerala, Animal Disease Control Project, Thrissur, India.

P. B. Giridas,
Department of Animal Husbandry-Kerala, Rinderpest Mobile Unit, Thrissur, India.

Latha Menon,
Department of Animal Husbandry-Kerala, District Veterinary Centre, Thrissur, India.

Vidhya Lakshmi Das,
Department of Animal Husbandry-Kerala, Animal Disease Control Project, Thrissur, India.

Please see the link here: https://stm.bookpi.org/CERB-V9/article/view/11177

Sunday, 26 February 2023

Molecular Evidence for the Presence of Borrelia burgdorferi and Anaplasma platys among Domesticated Animal Population-Study Based on Real Time PCR Methodology–in Thrissur District-Kerala, India | Chapter 4 | Newest Updates in Agriculture and Veterinary Science Vol. 3

 A very extreme prevalence of tick communicated diseases is observed between domesticated animal populace in Kerala now days. Conventional demonstrative methods established microscopy will not be sufficient to identify spirochete like Borrelia burgdorferi and germ like Anaplasma platys. The present study was aimed to use Taqman located qualitative Real Time PCR (qPCR) as an aid to think the presence of B. burgdorferi-bacterium creating Lyme disease and A. platys-germ affecting platelets, between our domesticated animal state. Previously reported, forward and reverse primers and probe sequences specific for A. platys (1) and B. burgdorferi (2) were secondhand in the study. Results of the present study shows that principal part the domesticated animal class tested from two together Wayanad and Thrissur districts were found to harbour either A. platys or Borrelia or two together pathogens. Based on the qualitative discovery of specific deoxyribonucleic acid targets, qPCR can be dependably used as an aid inaccurate and confirmatory disease up to class level. To authors knowledge this is the first report for molecular evidence of the A. platys and B. burgdorferi between domesticated animal public in the state of Kerala that should be a major concern and demands further detailed review including more number of samples from various districts describing different geographical domains.

Author(s) Details:

Sunitha Karunakaran,
Veterinary Dispensary, Koorkanchery, Department of Animal Husbandry, Thrissur, Kerala, India.

Gopika Thattat Gopinathan,
Clinical Laboratory, Animal Disease Control Project, Department of Animal Husbandry, Thrissur, Kerala, India.

Hareesh Sasidharan,
Clinical Laboratory, Animal Disease Control Project, Department of Animal Husbandry, Thrissur, Kerala, India.

Please see the link here: https://stm.bookpi.org/NUAVS-V3/article/view/9668