An Organic Solvent-Free and Space-Saving Method for Quantifying Cyromazine and Its Metabolite, Melamine, in Cow’s Milk | Chapter 2 | Recent Advances in Science and Technology Research Vol.3

The author described an organic solvent-free, rapid, simple, and space-saving method of sample
preparation followed by HPLC coupled photo-diode array (PDA) detector for simultaneous
quantification of cyromazine (CYR) and its metabolite, melamine (MEL), in milk. The HPLC-PDA was
performed on an Inertsil® HILIC column with an isocratic aqueous mobile phase. Analytes were
extracted from the sample using water, and purified by Mono-Spin®-C18, a centrifugal monolithic silica
spin mini-columns, and quantified within 20 min. The method, performed under 100% aqueous
conditions, obtained average recoveries for CYR and MEL in the range of 93.2%-99.1% with relative
standard deviations ≤ 2.8%. The quantification limits were 8.5 ng/mL for CYR and 10 ng/mL for MEL,
respectively. No organic solvents were used at any stage of the analysis.

Author (s) Details

Naoto Furusawa
Graduate School of Human Life Science, Osaka City University, Osaka, Japan.

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An Isocratic Water Mobile Phase HPLC Analysis of Melamine and Its Related Analogues | Chapter 15 | Current Research and Development in Chemistry Vol. 2

The author describes a reserved-phase high performance liquid chromatographic method for detecting melamine (MEL) and related analogues, cyanuric acid (CYA), ammeline (AML), and ammelide (AMD), using a 100% water mobile phase. Chromatographic separation was performed an Inertsil® ODS-4 (250 × 4.6 mm, 5 μm) with a water mobile phase and a photodiode-array detector. The monitoring wavelength was adjusted to 210 nm which represents an average maximum for all the analytes. The total run time was < 8 min. The method shows high stability, significant linearity and satisfactory sensitivity. The detection limits were <46 ng.mL–1. An inexpensive and harmless method for the simultaneous detection of MEL, CYA, AML, and AMD was developed and may be further applied to the quantification in foods.

Author(s) Details

Naoto Furusawa
Graduate School of Human Life Science, Osaka City University, Osaka, Japan.

View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/173