Standardization of An Efficient Micropropagation Protocol for Bacopa monnieri (L.) Pennell | Chapter 1 | Microbiology and Biotechnology Research: An Overview Vol. 6

 

Bacopa monnieri (L.) Pennell, commonly known as “Brahmi,” is a medicinally important plant widely used in Ayurveda for its neuroprotective and memory-enhancing properties. Despite its high medicinal value, large-scale cultivation of B. monnieri using conventional methods is limited by low seed viability, seasonal growth constraints, and habitat degradation. Micropropagation plays an important role in providing a practical solution by enabling the rapid production of disease-free, genetically uniform plantlets in a relatively short period. The present study aimed to standardise an efficient in vitro micropropagation protocol by optimising explant type, surface sterilisation, hormone concentration, and sucrose level. Nodal and leaf segments were selected as explants, sourced from the medicinal garden at the Onattukara Regional Agricultural Research Station, Kayamkulam. Effective surface sterilisation was achieved using 0.1% Bavistin for 30 minutes followed by 0.05% HgCl₂ for 4 minutes, which eliminated contamination while preserving viability. Shoot initiation occurred within 5–8 days, with the highest frequency of multiple shoots observed on MS (Murashige and Skoog) medium supplemented with 3 mg/L BA or 2 mg/L BA + 1 mg/L. Callus induction was most successful from leaf explants cultured on MS + 2 mg/L 2,4-D + 1 mg/L kinetin, forming friable and regenerative calli within 30–35 days. For rooting, IBA at 3 mg/L promoted direct multiple shoot and root formation, while MS + BAP (5.5 mg/L) + NAA (0.2 mg/L) supported both elongation and rooting. Optimal growth was obtained at pH 5.8 and 3% sucrose, which enhanced shoot number and explant vigour. The present study successfully developed an in vitro micropropagation protocol for Bacopa monnieri (L.) Pennell is using nodal and leaf explants. The developed protocol is fast, reliable, and reproducible, providing a basis for large-scale clonal propagation, conservation of germplasm, and potential metabolite enhancement in B. monnieri.

 

Author(s) Details

Jyothilekshmi S

Onattukara Regional Agricultural Research Station, Kayamkulam, Kerala Agricultural University, Kerala, India.

 

Sandra Krishnan
Onattukara Regional Agricultural Research Station, Kayamkulam, Kerala Agricultural University, Kerala, India.

 

Please see the link:- https://doi.org/10.9734/bpi/mbrao/v6/6606

In vitro Tuberization of Glory Lily (Gloriosa superba. L): Experimental Investigation| Chapter 12 | Cutting Edge Research in Biology Vol. 2

 Gloriosa superba L. (Colchicaceae) usually known as Kalahari and Glory lily was an export familiarize medicinal plant. Colchicine was the bigger tropalone alkaloids of medicinal importance that cures gout, tumor, rheumatism. Commercially propagated by tubers which are ‘V’ or ‘L’ formed, but was considered slow with weak multiplication percentage of 1:1 every year. The present study has described a doable procedure for artificial tuberization employing non-dormant tubers of Gloriosa superba L. complemented with variable quantities of auxin, cytokinin, and gibberellins on Murashige and Skoog medium. When sterilized with 70% intoxicating for 30 seconds and then 60 seconds in HgCl2, sprouted tuber bud explants had a contamination portion of only 8.00%. The maximum response for primary vegetable (100%) and secondary vegetable (100%) development was observed in MS medium enriched with 4.0 mgl-1 BAP and 1.0 mgl-1 NAA. This also allowed the most tubers (1.77) from a distinct explant. GA3 (1.0 mgl-1) was found to be essential for dash elongation, inasmuch as IAA (1.0 mgl-1) in combination with IBA (0.5 mgl-1) was direct for root induction on MS medium.Gloriosa superba L. (Colchicaceae) usually known as Kalahari and Glory lily was an export familiarize medicinal plant. Colchicine was the bigger tropalone alkaloids of medicinal importance that cures gout, tumor, rheumatism. Commercially propagated by tubers which are ‘V’ or ‘L’ formed, but was considered slow with weak multiplication percentage of 1:1 every year. The present study has described a doable procedure for artificial tuberization employing non-dormant tubers of Gloriosa superba L. complemented with variable quantities of auxin, cytokinin, and gibberellins on Murashige and Skoog medium. When sterilized with 70% intoxicating for 30 seconds and then 60 seconds in HgCl2, sprouted tuber bud explants had a contamination portion of only 8.00%. The maximum response for primary vegetable (100%) and secondary vegetable (100%) development was observed in MS medium enriched with 4.0 mgl-1 BAP and 1.0 mgl-1 NAA. This also allowed the most tubers (1.77) from a distinct explant. GA3 (1.0 mgl-1) was found to be essential for dash elongation, inasmuch as IAA (1.0 mgl-1) in combination with IBA (0.5 mgl-1) was direct for root induction on MS medium.

Author(s) Details:

Anandhi Selvarasu,
SRM College of Agricultural Sciences, Vendhar Nagar, Baburayanpettai, Acharapakkam-603 203; Chengalpattu (Dist.), India.

Rajamani Kandhasamy,
Department of Floriculture and Landscape Architecture, Horticultural College & Research Institute, Tamil Nadu Agricultural University, Coimbatore – 641 003, Tamil Nadu, India.

Please see the link here: https://stm.bookpi.org/CERB-V2/article/view/9050

Keywords: Glory lily, MS medium, tuber node, shooting, rooting, primary tubers