A variety of drugs are used in giardiasis treatment with
different levels of efficiency, presence of side effects, and even formation of
resistant strains, so that it is important to search new only-one-dose
treatments with high efficiency and less side effects. Kramecyne, an
anti-inflammatory compound isolated from methanolic extract of Krameria
cytisoides, does not present toxicity, even at doses of 5,000 mg/kg. The
objective was to determine the antigiardial effect of kramecyne over Giardia
intestinalis In vitro and In vivo and analyze the expression of genes ERK1,
ERK2, and AK on kramecyne treated trophozoites by Real Time Polymerase Chain
Reaction (RTPCR). The median lethal dose (LD50) was 40 g/mL and no
morphological changes were observed by staining with blue trypan and light
microscopy; experimental gerbil infection was eliminated with 320 g/Kg of
weight. After treatment there were no differences between intestines from
treated and untreated gerbils. Kramecyne did not have significant effect over
ERK1 and AK, but there are differences in ERK2 expression ( = 0.04). Results
show antigiardial activity of kramecyne; however the mode of action is still
unclear and the evaluation of ultrastructural damage and expressed proteins is
an alternative of study to understand the action mechanism.
Author(s) Details Leticia Eligio-García
Parasitology Research Laboratory, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Col. Doctores, Alcaldía Cuauhtémoc, 06720, Ciudad de México, México.
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