A Study on the Function of Laparoscopy to Identify the Etiology of Infertility | Chapter 9 | Novel Research Aspects in Medicine and Medical Science Vol. 1

 This study was attended to assess the act of diagnostic laparoscopy in the investigation of female unproductiveness and to evaluate the miscellaneous causes of infertility like endometriosis, PCOD tubal and peritoneal factors, uterine deviations, tuberculosis etc. by utilizing diagnostic laparoscopy. Although India's population growth is a weighty issue, infertility appears to affect 5- 15% of Indians. Each of these patients has expected assessed. Infertility maybe diagnosed with the use of laparoscopy. Following a complete dispassionate examination and point in a direction investigations, a demonstrative laparoscopy is carried out to look for semantic anomalies in the uterus, ovaries, and tuboperitoneum in addition to tube patency. The study involved 60 young patients the one visited the outpatient areas of the Tumkur hospitals owned by Sri Siddhartha Medical College. Patients with infertility, two together primary and subordinate, who underwent demonstrative laparoscopy and were eager to realize were assessed. The abscission was not performed on those who were reluctant or ineligible. 72% of subjects with basic unproductiveness and 28% of individuals accompanying secondary infertility had demonstrative laparoscopy. The majority of cases with initial unproductiveness were between the ages of 21 and 25; those accompanying secondary infertility were betwixt 26 and 30. The majority of victims in both groups had been young for 1 to 5 years. In our survey, the majority of the reasons of unproductiveness were due to tubal factors (50%) by some means. The rate of complications was reduced and comparable to previous prevailing research. The gold standard for identifying endometriosis, tubal and peritoneal sickness, and other pelvic pathologies is laparoscopic since no added imaging approach has the alike level of sensitivity or specificity. Laparoscopy for demonstrative purposes is therefore a critical technique in the assessment of unproductiveness.

Author(s) Details:

K. R. Hema,
Department of Obstetrics and Gynecology, Sree Sidhartha Medical College, Tumkur, Karnataka, India.

H. S. Lalitha,
Department of Obstetrics and Gynecology, Sree Sidhartha Medical College, Tumkur, Karnataka, India.

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Experiment and Investigation on the Correlation of Histological Dating and Glycogen Content by Histochemical Stain during Various Phases of Menstrual Cycle in Primary Infertility | Chapter 3 | Current Topics in Medicine and Medical Research Vol.2

Glycogen and its products of metabolism are considered to be the most important and direct source of
nutrient for the early conceptus. The premenstrual biopsies were taken from 100 women with primary
infertility whose male partners had normal semenograms. The biopsies were then subjected to
Haematoxylin and Eosin (H & E) and Periodic Acid Schiff (PAS) staining with and without diastase to
study histological and histochemical changes particularly glycogen content in the endometrium
respectively. Histolomorphologic spectrum of endometrium on H & E stained sections revealed early
proliferative phase (4%), late proliferative phase (7%) and early secretory phase (7%), mid secretory
phase (33%) and late secretory phase (49%). PAS staining with or without diastase for glycogen was
performed and glycogen content, as demonstrated by magenta color, was graded from + to ++++.
Glycogen content was observed to be “+” to “++” in proliferative phase, where as in secretory phase, it
varied from “++” to “+++”. Glycogen was confirmed by treatment with diastase enzyme, after that PAS
stain gave negative staining, i.e. loss of magenta color as glycogen was digested by diastase.
Glycogen deficiency was observed in 24.7% of secretory phase endometrium of infertile women.
Depletion of glycogen results in inadequate preparation of endometrium at the time of implantation,
and hence, may be one of the causes of infertility.

Author(s) Details

Dr. Anshu Gupta
Department of Pathology, Institute of Human Behaviour & Allied Sciences (IHBAS), Delhi, India.

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