The Lichen Substances of Ramalina hossei Vain.with Their Anthelmintic and Antibacterial Studies | Chapter 2 | Recent Research Advances in Biology Vol. 1

 

Lichens are well known for their peculiar, crystalline aromatic constituents that accumulate on the

thallus. Lichen compounds are also known to show some biological activities against microorganisms.

The present paper describes the extraction and antibacterial activity of various solvent extracts of

lichen Ramalina hossei Vain. (Ramalinaceae), against Gram positive and Gram negative bacteria.

The dried and powdered lichen material was extracted with solvents namely methanol, chloroform and

petroleum ether and the solvent extracts were subjected to antibacterial activity using Kirby Bauer

method. Chemical tests revealed the presence of Usnic acid and sekikaic acid aggregates. The

extracts were found to possess more activity against Gram positive bacteria than Gram negative

bacteria. Among solvent extracts, methanol extract showed more inhibition of test bacteria than

others. From the results, it could be concluded that the lichen selected for the study possess active

antimicrobial principles. The results are in justification with the folkloric uses of lichen extracts.

Author (s) Details

K. S. Vinayaka

Plant Biology Lab., Department of Botany, Sri Venkataramana Swamy College, Vidyagiri, Bantwal-574211, Dakshina Kannada, Karnataka, India.

T. R. Prashith Kekuda

Department of Microbiology, S.R.N.M.N College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India.

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Anticaries Activity of Lichen Species Usnea pictoides from Karnataka, India | Chapter 11 | Emerging Issues in Science and Technology Vol. 4

Lichens form an important floral community, playing an important role in ecological succession. The Western Ghats harbour a number of plant resources including lichens, mosses and other lower plants. The present study was conducted to determine anticaries activity of solvent extracts of a macrolichen Usnea pictoides G. Awasthi (Parmeliaceae) collected at Mullayanagiri, Western Ghats of Chikmagalur district, Karnataka, India. The lichen material was sequentially extracted using solvents viz., petroleum ether, ethyl acetate, chloroform and methanol in a soxhlet assembly. Anticaries activity of solvent extracts was determined against four clinical isolates of Streptococcus mutans (recovered from dental caries subjects) by Agar well diffusion assay. All solvent extracts were effective against the clinical isolates. High inhibitory potential was observed in case of chloroform extract. Thin layer chromatogram showed the presence of Usnic acid. Inhibitory effect could be ascribed to the bioactive secondary metabolites, mainly Usnic acid present in the lichen. Purification of bioactive principles and determination of their anticaries activity are to be conducted. 

Author (s) Details

Dr.  K. S. Vinayaka
Plant Science Laboratory, Department of Botany, Sri Venkataramana Swamy College, Vidyagiri, Bantwal-574211, Dakshina Kannada, Karnataka, India.

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