Objectives: The purpose of this study was to evaluate the ability
of vitamin E and polyphenols (red wine polyphenols, resveratrol, and catechin)
on fluidity (fluorescence anisotropy) and anion permeability for chloride of
membrane on mammalian erythrocytes respectively exposed to the water-soluble
free radical initiator 2.2’-azobis-2 amidinopropan dihydrochloride (AAPH) and
trained horses.
Methods: The animals were ten Arabian strain horses, 6-8 years
old, trained for Cross country, and six horses at rest. Membranes are tested
for diphenylhexatriene fluorescence anisotropy in red blood cell membranes by
Perrin plot.
The reducing agent treatment efficacy was observed by the
evaluation of anion permeability for chloride in red blood cells (RBCs).
Anion permeability for chloride is an indicator of membrane
protein damage and is evaluated in RBCs by the specific absorption of
methemoglobin (CM) at 590 and 635 nm after treatment of heparinized blood with
NaNO2.
Results: Fluorescence anisotropy is abruptly worsened in
erythrocyte membranes from exercised animals and almost restored after vitamin
E treatment of membrane preparations and P > 0.05 by ANOVA.
Polyphenol treatment is associated with a significant increase in
anion permeability for chloride compared with control and AAPH-affected cells.
Discussion: Fluorescence anisotropy is abruptly worsened in
erythrocyte membranes from exercised animals and almost restored after vitamin
E treatment of membrane preparations.
In this study, it is evident that after In vitro oxidative damage
of the membrane, red wine polyphenol extracts act as effective reducing agents
and also as a CM indicator of membrane protein damage. Consequently, both red
wine and polyphenol extracts both alone and mixed among them efficiently relieve
the effects of oxidative stress.
Author(s)
Details
Giuseppe
Gallo
Department of Biology, Ecology and Earth Sciences, Laboratory of
Cell Physiology, University of Calabria,
Rende (CS) 87036, Italy.
Please see the book here:- https://doi.org/10.9734/bpi/ibs/v10/1947
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