Background: Cervical and lung cancer cases increased
worldwide as well as in Indonesia from 2018 to 2020. Cancer treatment often
involves multidisciplinary approaches and is uncomfortable. Therefore,
alternative medicine from natural ingredients is needed. Previous studies
showed that the Cantigi leaf ethyl acetate extract has potent cytotoxic
activity on L1210 leukaemia cells (IC50 of 8.29 ppm), on MCF-7 cells (IC50 of
75.23 ppm) and T47D cells (IC50 of 88.89 ppm), but no reports exist on HeLa and
A549 cells. The side effects of cancer therapies cause painful and
uncomfortable conditions; therefore, complementary and alternative medicine
(CAM), including herbal medicine, have become common medical products and
practices.
Objective: The present study aimed to determine the
cytotoxic activity of the ethyl acetate extract of Cantigi leaf on HeLa cells
and A549 cells.
Methods: Powdered dry young Cantigi leaves were macerated
with hexane, followed by ethyl acetate. After vacuum evaporation, the dry
extract was characterised and tested for its cytotoxic activity on HeLa cells
and A549 cells using the MTT method.
Results: The ethyl acetate extract colour was dark green
with a distinctive Cantigi odour; it had a water content of 4.3% and an acidic
pH of 2.87. It showed functional groups of O-H, C-H (aliphatic), C-H
(hydrocarbon), C=N, C=O, and C=C. Finally, the IC50 of the ethyl acetate
extract on A549 cells was 74.74±5.29 ppm, while on the HeLa cells, it was
79.69±2.54 ppm. The IC50 value of the ethyl acetate extract of Cantigi leaves
for each cancer cell can be determined by changing the graph of the extract
concentration versus the percentage of viability of cancer cells into a log of
the extract concentration versus the percentage of inhibition of cancer cells.
Conclusion: The ethyl acetate extract of Cantigi leaves
showed potent in vitro cytotoxic activity on HeLa cervical cancer cells and
A549 lung cancer cells.
Author(s) Details
Kosasih Kosasih
Fakultas Farmasi, Universitas Pancasila, Jakarta, Indonesia.
Hasna Nurfitriyati
Fakultas Farmasi, Universitas Pancasila, Jakarta, Indonesia.
Reza Hafidz
Fakultas Farmasi, Universitas Pancasila, Jakarta, Indonesia.
Please
see the book here:- https://doi.org/10.9734/bpi/prrat/v5/1528
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